| Title: | An immune-associated mitochondrial DNA variant with sex differences reveals a putative novel microprotein called MASL |
| Journal: | Immunity & Ageing |
| Published: | 23 Jun 2026 |
| Pubmed: | https://pubmed.ncbi.nlm.nih.gov/42337615/ |
| DOI: | https://doi.org/10.1186/s12979-026-00576-6 |
Publication 20554
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Abstract
The use of mitochondrial wide association studies (MiWAS) to link mitochondrial DNA variants (mtSNPs) to phenotypes of interest has uncovered important connections between mitochondrial genes and human health. The recent introduction of a re-annotated mitochondrial genome that accounts for small open reading frames (sORFs) with protein coding potential suggests the existence of mitochondrial-derived microproteins, many of which remain uncharacterized. Thus, considering the re-annotated mitochondrial genome when conducting genomic analyses such as MiWAS facilitates the mapping of mtSNPs back to microprotein-encoding sORFs and uncovers interactions between mitochondrial microproteins and biological systems. Here, we employ MiWAS of venous blood samples from the Health and Retirement Study (HRS) and identify a mtSNP associated with sex-specific changes to immune composition. After accounting for re-annotation, we map the identified mtSNP back to a sORF that encodes a novel microprotein, termed MASL (Mitochondrial Associated Small d-Loop peptide). Complementary phenome-wide association studies (PheWAS) in HRS and and UK Biobank confirm interactions between this mtSNP and immune phenotypes of interest, and our targeted RNA-Seq method (mitoSNP-seq) elucidates sex-differences in gene expression and functional pathways potentially altered by this mtSNP that may be relevant to the associated microprotein. Early characterization of the MASL microprotein shows sex-differences in circulating MASL levels in human plasma, and sex-specific interactions when comparing male and female mice treated with synthesized MASL. Together, the results of this study not only contribute to our understanding of mitochondrial dynamics in immunity, but also provide early characterization of a novel mitochondrial-derived microprotein with sex-specific modulatory effects.</p>
14 Authors
- Ana R. Silverstein
- Thalida E. Arpawong
- Eileen M. Crimmins
- Tae Jung Oh
- Rong Lu
- Melanie Flores
- Hiroshi Kumagai
- Junxiang Wan
- Hemal H. Mehta
- Kelvin Yen
- Brendan Miller
- Yeachan Lee
- Anna Nogalska
- Pinchas Cohen
1 Application
| Application ID | Title |
|---|---|
| 66866 | Genomic Translation of Aging-Related Associations from Model Systems to Humans |
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