| Title: | Unveiling inflammatory and prehypertrophic cell populations as key contributors to knee cartilage degeneration in osteoarthritis using multi-omics data integration |
| Journal: | Annals of the Rheumatic Diseases |
| Published: | 7 Feb 2024 |
| Pubmed: | https://pubmed.ncbi.nlm.nih.gov/38325908/ |
| DOI: | https://doi.org/10.1136/ard-2023-224420 |
| URL: | https://ard.bmj.com/content/annrheumdis/early/2024/02/07/ard-2023-224420.full.pdf |
Publication 8792
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Abstract
OBJECTIVES: Single-cell and spatial transcriptomics analysis of human knee articular cartilage tissue to present a comprehensive transcriptome landscape and osteoarthritis (OA)-critical cell populations.</p>
METHODS: Single-cell RNA sequencing and spatially resolved transcriptomic technology have been applied to characterise the cellular heterogeneity of human knee articular cartilage which were collected from 8 OA donors, and 3 non-OA control donors, and a total of 19 samples. The novel chondrocyte population and marker genes of interest were validated by immunohistochemistry staining, quantitative real-time PCR, etc. The OA-critical cell populations were validated through integrative analyses of publicly available bulk RNA sequencing data and large-scale genome-wide association studies.</p>
RESULTS: We identified 33 cell population-specific marker genes that define 11 chondrocyte populations, including 9 known populations and 2 new populations, that is, pre-inflammatory chondrocyte population (preInfC) and inflammatory chondrocyte population (InfC). The novel findings that make this an important addition to the literature include: (1) the novel InfC activates the mediator MIF-CD74; (2) the prehypertrophic chondrocyte (preHTC) and hypertrophic chondrocyte (HTC) are potentially OA-critical cell populations; (3) most OA-associated differentially expressed genes reside in the articular surface and superficial zone; (4) the prefibrocartilage chondrocyte (preFC) population is a major contributor to the stratification of patients with OA, resulting in both an inflammatory-related subtype and a non-inflammatory-related subtype.</p>
CONCLUSIONS: Our results highlight InfC, preHTC, preFC and HTC as potential cell populations to target for therapy. Also, we conclude that profiling of those cell populations in patients might be used to stratify patient populations for defining cohorts for clinical trials and precision medicine.</p>
13 Authors
- Yue Fan
- Xuzhao Bian
- Xiaogao Meng
- Lei Li
- Laiyi Fu
- Yanan Zhang
- Long Wang
- Yan Zhang
- Dalong Gao
- Xiong Guo
- Mikko Juhani Lammi
- Guangdun Peng
- Shiquan Sun
1 Application
| Application ID | Title |
|---|---|
| 67665 | Developing scalable computational tools for understanding polygenic trait architectures in large-scale genome-wide association studies |
Enabling scientific discoveries that improve human health