| Title: | Mitochondrial genome copy number measured by DNA sequencing in human blood is strongly associated with metabolic traits via cell-type composition differences |
| Journal: | Human Genomics |
| Published: | 7 Jun 2021 |
| Pubmed: | https://pubmed.ncbi.nlm.nih.gov/34099068/ |
| DOI: | https://doi.org/10.1186/s40246-021-00335-2 |
| URL: | https://escholarship.org/uc/item/723919sn |
| Citations: | 12 (6 in last 2 years) as of 8 Aug 2024 |
Publication 5403
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Abstract
BackgroundMitochondrial genome copy number (MT-CN) varies among humans and across tissues and is highly heritable, but its causes and consequences are not well understood. When measured by bulk DNA sequencing in blood, MT-CN may reflect a combination of the number of mitochondria per cell and cell-type composition. Here, we studied MT-CN variation in blood-derived DNA from 19184 Finnish individuals using a combination of genome (N = 4163) and exome sequencing (N = 19034) data as well as imputed genotypes (N = 17718).ResultsWe identified two loci significantly associated with MT-CN variation: a common variant at the MYB-HBS1L locus (P = 1.6 × 10−8), which has previously been associated with numerous hematological parameters; and a burden of rare variants in the TMBIM1 gene (P = 3.0 × 10−8), which has been reported to protect against non-alcoholic fatty liver disease. We also found that MT-CN is strongly associated with insulin levels (P = 2.0 × 10−21) and other metabolic syndrome (metS)-related traits. Using a Mendelian randomization framework, we show evidence that MT-CN measured in blood is causally related to insulin levels. We then applied an MT-CN polygenic risk score (PRS) derived from Finnish data to the UK Biobank, where the association between the PRS and metS traits was replicated. Adjusting for cell counts largely eliminated these signals, suggesting that MT-CN affects metS via cell-type composition.ConclusionThese results suggest that measurements of MT-CN in blood-derived DNA partially reflect differences in cell-type composition and that these differences are causally linked to insulin and related traits.</p>
21 Keywords
- Adult
- Aged
- Apoptosis Regulatory Proteins
- Cell Lineage
- DNA Copy Number Variations
- DNA, Mitochondrial
- Exome Sequencing
- Female
- GTP-Binding Proteins
- Genetic Predisposition to Disease
- Genome, Mitochondrial
- Genome-Wide Association Study
- Humans
- Male
- Membrane Proteins
- Mendelian Randomization Analysis
- Middle Aged
- Phenotype
- Polymorphism, Single Nucleotide
- Proto-Oncogene Proteins c-myb
- Sequence Analysis, DNA
24 Authors
- Liron Ganel
- Lei Chen
- Ryan Christ
- Jagadish Vangipurapu
- Erica Young
- Indraniel Das
- Krishna Kanchi
- David Larson
- Allison Regier
- Haley Abel
- Chul Joo Kang
- Alexandra Scott
- Aki Havulinna
- Charleston W. K. Chiang
- Susan Service
- Nelson Freimer
- Aarno Palotie
- Samuli Ripatti
- Johanna Kuusisto
- Michael Boehnke
- Markku Laakso
- Adam Locke
- Nathan O. Stitziel
- Ira M. Hall
Enabling scientific discoveries that improve human health