| Title: | Endothelial force sensing signals to parenchymal cells to regulate bile and plasma lipids |
| Journal: | Science Advances |
| Published: | 27 Sep 2024 |
| Pubmed: | https://pubmed.ncbi.nlm.nih.gov/39331703/ |
| DOI: | https://doi.org/10.1126/sciadv.adq3075 |
Publication 13322
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Abstract
How cardiovascular activity interacts with lipid homeostasis is incompletely understood. We postulated a role for blood flow acting at endothelium in lipid regulatory organs. Transcriptome analysis was performed on livers from mice engineered for deletion of the flow-sensing PIEZO1 channel in endothelium. This revealed unique up-regulation of Cyp7a1, which encodes the rate-limiting enzyme for bile synthesis from cholesterol in hepatocytes. Consistent with this effect were increased gallbladder and plasma bile acids and lowered hepatic and plasma cholesterol. Elevated portal fluid flow acting via endothelial PIEZO1 and genetically enhanced PIEZO1 conversely suppressed Cyp7a1. Activation of hepatic endothelial PIEZO1 channels promoted phosphorylation of nitric oxide synthase 3, and portal flow-mediated suppression of Cyp7a1 depended on nitric oxide synthesis, suggesting endothelium-to-hepatocyte coupling via nitric oxide. PIEZO1 variants in people were associated with hepatobiliary disease and dyslipidemia. The data suggest an endothelial force sensing mechanism that controls lipid regulation in parenchymal cells to modulate whole-body lipid homeostasis.</p>
16 Keywords
- Animals
- Bile
- Bile Acids and Salts
- Cholesterol
- Cholesterol 7-alpha-Hydroxylase
- Endothelial Cells
- Endothelium
- Hepatocytes
- Humans
- Ion Channels
- Lipid Metabolism
- Lipids
- Liver
- Mice
- Nitric Oxide
- Nitric Oxide Synthase Type III
17 Authors
- Laeticia Lichtenstein
- Chew W Cheng
- Muath Bajarwan
- Elizabeth L Evans
- Hannah J Gaunt
- Fiona Bartoli
- Eulashini Chuntharpursat-Bon
- Shaili Patel
- Charalampos Konstantinou
- T Simon Futers
- Melanie Reay
- Gregory Parsonage
- J Bernadette Moore
- Justine Bertrand-Michel
- Piruthivi Sukumar
- Lee D Roberts
- David J Beech
1 Application
| Application ID | Title |
|---|---|
| 42651 | Single nuclear polymorphisms in PIEZO1 gene and Cardiovascular Diseases |
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