| Title: | Single-cell RNA sequencing reveals time- and sex-specific responses of mouse spinal cord microglia to peripheral nerve injury and links ApoE to chronic pain |
| Journal: | Nature Communications |
| Published: | 11 Feb 2022 |
| Pubmed: | https://pubmed.ncbi.nlm.nih.gov/35149686/ |
| DOI: | https://doi.org/10.1038/s41467-022-28473-8 |
| URL: | https://www.nature.com/articles/s41467-022-28473-8.pdf |
| Citations: | 79 (58 in last 2 years) as of 8 Aug 2024 |
Publication 12536
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Abstract
Activation of microglia in the spinal cord following peripheral nerve injury is critical for the development of long-lasting pain hypersensitivity. However, it remains unclear whether distinct microglia subpopulations or states contribute to different stages of pain development and maintenance. Using single-cell RNA-sequencing, we show that peripheral nerve injury induces the generation of a male-specific inflammatory microglia subtype, and demonstrate increased proliferation of microglia in male as compared to female mice. We also show time- and sex-specific transcriptional changes in different microglial subpopulations following peripheral nerve injury. Apolipoprotein E (Apoe) is the top upregulated gene in spinal cord microglia at chronic time points after peripheral nerve injury in mice. Furthermore, polymorphisms in the APOE gene in humans are associated with chronic pain. Single-cell RNA sequencing analysis of human spinal cord microglia reveals a subpopulation with a disease-related transcriptional signature. Our data provide a detailed analysis of transcriptional states of mouse and human spinal cord microglia, and identify a link between ApoE and chronic pain in humans.</p>
14 Keywords
- Animals
- Apolipoproteins E
- Cell Proliferation
- Chronic Pain
- Female
- Gene Expression
- Male
- Mice
- Mice, Inbred C57BL
- Microglia
- Peripheral Nerve Injuries
- Polymorphism, Genetic
- Sequence Analysis, RNA
- Spinal Cord
23 Authors
- Shannon Tansley
- Sonali Uttam
- Alba Ureña Guzmán
- Moein Yaqubi
- Alain Pacis
- Marc Parisien
- Haley Deamond
- Calvin Wong
- Oded Rabau
- Nicole Brown
- Lisbet Haglund
- Jean Ouellet
- Carlo Santaguida
- Alfredo Ribeiro-da-Silva
- Soroush Tahmasebi
- Masha Prager-Khoutorsky
- Jiannis Ragoussis
- Ji Zhang
- Michael W. Salter
- Luda Diatchenko
- Luke M. Healy
- Jeffrey S. Mogil
- Arkady Khoutorsky
1 Application
| Application ID | Title |
|---|---|
| 20802 | A replication study of pain interactions with comorbidities |
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